Comparison of Two Kinds of Automated Microbial Identification System Used in the Identification of Brucella
10.3969/j.issn.1671-7414.2015.04.030
- VernacularTitle:两种全自动微生物鉴定系统在布鲁菌鉴定中的应用比较
- Author:
Minggao XU
;
Jinya DING
;
Juan XU
- Publication Type:Journal Article
- Keywords:
automated microbial identification system;
brucella;
identification;
16S rRNA gene sequence
- From:
Journal of Modern Laboratory Medicine
2015;(4):105-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare Vitek2 Compact and Walkaway 40 in the identification of Brucella.Methods Used Vitek2 Compact and Walkaway 40 automated microbial identification system to identify clinical isolates and compared with the de-tection of 16S rRNA gene sequences analysis.Results The clinical isolates was identified as Bergeyella zoohelcum or Moraxella by Walkaway 40 and as Brucella melitensis by Vitek2 Compact.16S RNA sequence analysis of the isolate,the se-quence was identical to the sequences of 16S rRNA of Brucella,which excluded the possibility of B.zoohelam and Moraxel-la .Determined that the isolate was B.melitensis.Conclusion Vitek 2 Compact can accurately identified Brucella.Use molec-ular methods to corroborate when the isolates was identified as Brucella by Vitek 2 Compact,this method can greatly im-prove the detection rate of brucellosis and reduce the possibility of misdiagnosis.Walkaway 40 cannot accurately identified Brucella,Misidentification of Brucella can result in wrong treatment of the patient and let the staff in the risk of laboratory-acquired infection.Recommend laboratory should be cautious reporting in the identified B.zoohelam or Moraxella by Walk-away 40 and use Vitek2 Compact or molecular methods for review.
