Optimum extraction of polysaccharides from ophiopogon japonicus by response surface methodology
10.3760/cma.j.issn.1673-4246.2015.10.014
- VernacularTitle:响应面分析法优化麦冬多糖的提取工艺
- Author:
Jingwen ZHAO
;
Min WANG
;
Chuanjian LU
;
Zhiren WANG
;
Wei ZHU
- Publication Type:Journal Article
- Keywords:
Ophiopogon japonicus;
Polysaccharides;
Response surface methodology;
Extraction
- From:
International Journal of Traditional Chinese Medicine
2015;37(10):914-919
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction of polysaccharides from Ophiopogon japonicus by the use of response surface methodology.Method According to the design principle of Box-Behnken, effects of extraction temperature, extraction time , extraction times and solid-liquid ratio on the extraction yield of Ophiopogon japonicus polysaccharides were studied by response surface methodology,meanwhile regression analysis of these experimental data was used by Design-Expert 8.05b software.Results The optimum extraction conditions from Ophiopogon japonicus were concluded as follows: the extraction temperature was 93℃, extraction time was 2 h, extraction times was 3 times, and solid-liquid ratio was 1:15 g/ml.Conclusion Under these conditions, the extraction yield of Ophiopogon japonicus polysaccharides is up to 5.440%, and the relative error is 7.328%.
