Application of leukoreduction filter in removal of leukocytes in platelet concentrate
10.3969/j.issn.1673-4130.2015.17.023
- VernacularTitle:血小板型去白细胞滤器在浓缩血小板去白细胞中的应用
- Author:
Wenhui HU
;
Lixian RUAN
;
Haitao CHENG
;
Yanshun WEI
- Publication Type:Journal Article
- Keywords:
platelet;
leukoreduction filter;
leukocytes;
platelet hypotonic shock;
platelet aggregation
- From:
International Journal of Laboratory Medicine
2015;(17):2509-2511
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application of leukoreduction filter in removal of leukocytes in platelet concentrate . Methods Platelet concentrate was prepared by using platelet‐rich plasma method .35 bags of the same type of prepared platelet concentrate were filtered by using leukoreduction filter .The changes of conventional indicators of platelet before and after filter were measured and recorded .The activating platelets indicators PAC‐1 and CD62p were detected by using flow cytometry .The platelet hypotonic shock was measured by biochemical analyzer .The platelet aggregation was measured by using platelet aggrega‐tion instrument .Results After platelet concentrate was filtered by using leukoreduction filter ,leukocyte removal rate was(98 .28 ± 0 .97)% ,platelet recovery rate was(86 .37 ± 2 .84)% .After filtration ,white blood cell count ,platelets ,red blood cells were reduced than before filtration(P<0 .05) .The differences of capacity of platelets ,mean platelet volume and pH before and after filtration were not statistically significant(P> 0 .05) .Before and after filtration ,the expression of platelet activation markers PAC‐1 and CD62p ,platelet aggregation and platelet hypotonic shock were not statistically different(P>0 .05) .Conclusion Platelet leukore‐duction filter could effectively filter leukocytes in platelet concentrate .It would not change the conventional indicators ,and not affect platelet activation ,aggregation and anti‐hypotonic shock capacity significantly .
