The application of EP14-A3 in the evaluation of endogenous antibodies interference in immunoassay reagent
10.3969/j.issn.1673-4130.2015.17.033
- VernacularTitle:E P14-A 3文件在试剂内源性抗体干扰研究中的应用
- Author:
Juan ZHANG
;
Qing WEI
;
Junjun LIU
- Publication Type:Journal Article
- Keywords:
immunoassay reagent;
endogenous antibodies interference;
EP14-A3
- From:
International Journal of Laboratory Medicine
2015;(17):2533-2535
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the deficiencies of current methods ,explore a new way to estimate endogenous antibodies in‐terference in immunoassay reagent .Methods According to EP14‐A3 ,RF samples and normal samples were tested at the same time by reference reagents ,reagent A and B respectively .Reagent A and B were to be evaluated .RF samples′location was compared to 95% CI of Deming regression line based on the normal samples .Results In comparison of reagent A vs .reference reagent ,RF sam‐ples exceeded 95% CI upper limit ,which indicated the anti‐interference ability to RF of reagent A was different from the reference reagent statistically .Meanwhile ,all RF samples tested by reagent B fell in 95% CI ,RF samples interfered reagent B hardly ,which indicated the reagent B had similar anti‐interference performance to RF as reference reagent .Conclusion The method from EP14‐A could intuitively reflect the resistance to endogenous antibodies for newly developed immunoassay reagents .
