Expression of MMP-9 protein and its mRNA in the tissues of breast cancer
10.3969/j.issn.1673-4130.2015.17.001
- VernacularTitle:MMP-9蛋白及mRNA在乳腺癌组织中的表达
- Author:
Haixia LIU
;
Xinmin NIE
;
Jun YANG
- Publication Type:Journal Article
- Keywords:
breast cancer;
matrix metalloproteinase-9;
immunohistochemistry;
reverse transcription polymerase chain re-action
- From:
International Journal of Laboratory Medicine
2015;(17):2457-2458
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study expression of matrix metalloproteinase‐9(MMP‐9) protein and its mRNA in breast cancer and e‐valuate its significance in the occurrence ,development and metastasis of breast cancer .Methods The protein expression of MMP‐9 breast cancer were detected by using immunohistochemistry and the expression of mRNA were detected by reverse transcription polymerase chain reaction(RT‐PCR)Results The positive rate of MMP‐9 protein expression in 56 cases of breast cancer was 69 .6% (39/56) ,while in benign breast diseases was 20% (6/30) ,which were significantly different(P<0 .05) .The levels of MMP‐9 mRNA were significantly higher in the patients with breast cancer than those in benign breast diseases(P<0 .05) ,which were 0 .914 2 ± 0 .108 1 and 0 .379 4 ± 0 .0428 respectively .Conclusion The MMP‐9 protein and mRNA expression in human breast cancers are positively correlated with the stage and lymph node metastasis .Expression of MMP‐9 could be used as an indicator for the ability of invasion and metastasis of breast cancer .
