The detection of CD44v2 expression by using real-time fluorescent quantitative PCR in bladder and urothelial carcinoma
10.3969/j.issn.1673-4130.2015.17.010
- VernacularTitle:实时荧光定量 PCR检测CD44v2在膀胱及尿路上皮癌中的表达
- Author:
Xiaorong LIU
;
Yongxiang WANG
;
Liming ZHAO
;
Xiaodong ZHAO
;
Xuejun LIU
;
Yulin REN
;
Shaojun LI
- Publication Type:Journal Article
- Keywords:
bladder and urothelial carcinoma;
real-time fluorescent quantitative PCR;
CD44 variant
- From:
International Journal of Laboratory Medicine
2015;(17):2478-2479
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of CD44 varant 2(CD44v2) in bladder and urothelial carcinoma ,and to study its significance in the diagnosis of human bladder and urothelial carcinoma .Methods Real‐time fluorescent quantitative PCR was used to analyze the expression of CD44v2 protein in 70 bladder and urothelial carcinoma tissue samples from patients in different pathological and clinical stages .Meanwhile ,20 tissue samples of normal bladder mucosa were collected as controls .Results CD44v2 expression was negative in normal bladder and urothelial mucosa ,the gene copies were less than 1 × 102 copies/mL ,while the posi‐tive expression rate of CD44v2 was 42 .9% (30/70) ,and Ct values were less than 35 and copy number was greater than 1 × 104 cop‐ies/mL .Positive expression rate was correlated with high pathological grades and TNM stages .Conclusion CD44v2 could be an useful indicator for the early assessment of bladder and urothelial carcinoma .
