Simultaneous HPLC determination of 9 effective components ofTanreqing injection
10.3760/cma.j.issn.1673-4246.2015.09.013
- VernacularTitle:高效液相色谱法同时测定痰热清注射液中9种有效成分
- Author:
Yonggang CHEN
;
Shiqiang WU
;
Chuanxue MA
;
Bing HU
- Publication Type:Journal Article
- Keywords:
HPLC;
Tanreqing injection;
Effective component
- From:
International Journal of Traditional Chinese Medicine
2015;37(9):817-821
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC method for the simultaneous detgrmination of 9 effective components ofTanreqing injection.Methods The Agilent TC-C18 (2) (250 mm×4.6 mm, 5μm) column was used, the mobile phase consisted of acetonitrile (A) acetonitrile-0.1% formic acid aqueous solution (B) in a gradient mode, the flow rate was 1.0 ml/min,the column temperature was 30℃, the injection volume was 10μl, and the detecting wavelength was at 280 nm.Results All the 9 effective components showed a good linear relationship. TheRSDof the precision, reproducibility and stability tests were less than 2%. The average recoveries of the 9 effective components were in the range of 99.3%-103.1%.Conclusion This analysis method is simple, accurate and has a good specificity, which can be suitable for controlling the quality of Tanreqing injection.
