Development of practical and low-cost instrument for protein purification
10.7687/J.ISSN1003-8868.2015.09.031
- VernacularTitle:一种低成本蛋白质监测与控制仪器装置研制
- Author:
Xinhua MA
;
Xiaoli LI
;
Xianjun FAN
;
Guorong OU
;
Nan LIU
;
Zhixian GAO
- Publication Type:Journal Article
- Keywords:
simple;
supervision of protein purification;
collection of purified protein solution;
Wince embedded system
- From:
Chinese Medical Equipment Journal
2015;(9):31-33
- CountryChina
- Language:Chinese
-
Abstract:
To develop a low-cost, reliable, easy-to-maintain and practical instrument for protein purification. Some ultraviolet luminescent diode was used to provide 280 nm light source, and high-sensitivity S1336 photo-electric detector was employed for real-time monitoring of purified protein solution flowing through quartz cell to supervise the concentration of the protein. The instrument gave voice alarm and stopped working in case the protein concentration was less than the standard one. The lower SCM monitored the liquid level of the protein collecting cup and the position of loading arm through laser infrared distance sensor, so that a cup full of protein might be replaced by another empty cup. The instrument involved in Samsung S5PV210 embedded master computer, Wince6.0 operating system, Keil4.0 and VS2005. Trials proved that the instrument could perform real-time monitoring and curve display of dual-channel ultraviolet absorption, and could realize auto collection of 735 ml protein solution and up to 5-hour standby. The instrument developed has simple structure, high reliability and easy maintenance, and meets the desired require-ments.
