Determination of molecular weight and weight distribution in Iron Maltose Syrup by HPGPC
- VernacularTitle:HPGPC法测定麦芽糖铁糖浆分子量与分子量分布
- Author:
Xuwen JIAO
;
Weiyang LIANG
- Publication Type:Journal Article
- Keywords:
HPGPC;
Iron Maltose Syrup;
molecular weight;
molecular weight distribution
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;35(10):131-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining the molecular weight (Mw) and molecular weight distribution of Iron Maltose Syrup. Methods HPGPC was used; PSS HEMA was used as column.Detector was differential refraction detector.Mobile phase was phosphate buffer solution (pH6.8) at 0.5 mL/min, column temperature was 45℃.Results The Mw of 3 batches of Iron Maltose Syrup were 45000-47000 Da with good linearity, precision and reproducibility.Conclusion The method is simple, reliable and accepted by the specification for controlling the molecular weight and weight distribution of Iron Maltose Syrup.
