Epidermal growth factor receptor expression and KRAS and BRAF mutations in sinonasal intestinal-type adenocarcinomas
10.16066/j.1672-7002.2015.11.008
- VernacularTitle:鼻腔鼻窦肠型腺癌中表皮生长因子受体蛋白表达及相关基因突变分析
- Author:
Jianying NIU
;
Yong CHEN
;
Pingdong LI
- Publication Type:Journal Article
- Keywords:
Receptor,Epidermal Growth Factor;
Genes;
Mutation;
Immunohistochemistry;
sinonasal intestinal-type adenocarcinoma
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2015;(11):569-573
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the expression and clinical significance of epidermal growth factor receptor (EGFR), the mutations of gene EGFR, KRAS and BRAF in sinonasal intestinal-type adenocarcinomas. METHODS We investigated the EGFR protein expression by immunohistochemistry method with antibodies targeting the extracellular domain, the intracellular domain, and the phosphorylated isoform in a series of 11sinonasal intestinal-type adenocarcinomas tissues. EGFR, KRAS, and BRAF mutational status were detected by DNA direct sequencing and Melt curve method.RESULTS The findings were analyzed with respect to clinical data, histological typing, and outcome of the patients. EGFR was expressed in 72.7% tumors with a focal distribution with both extracellular domain and intracellular domain, which showed a significantly correlation. p-EGFR was expressed in 9.1% tumors. 9.1% tumors had the mutation of EGFR gene in exon 19, 9.1% tumors had the mutation in exon 2 and exon 15 of gene KRAS and BRAF respectively. There was no association between these molecular features and the survival period of the patients. CONCLUSION The current study revealed various EGFR expression patterns in sinonasal intestinal-type adenocarcinomas, that indicated the tumor heterogeneity. Sinonasal intestinal-type adenocarcinomas share common alterations of the EGFR pathway as the mutation of EGFR, KRAS and BRAF genes, but with a lower frequency.
