Immunohistochemical study of 14-3-3-sigma and -zeta in ovarian cancer.
- Author:
Ji Kwon PARK
1
;
Jeong Kyu SHIN
;
Won Jun CHOI
;
Soon Ae LEE
;
Jong Hak LEE
;
Won Young PAIK
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National university, JinJu, Korea. wypaik@gshp.gsnu.ac.kr
- Publication Type:Original Article
- Keywords:
14-3-3sigma;
14-3-3zeta;
Ovarian epithelial carcinoma;
Immunohistochemistry
- MeSH:
14-3-3 Proteins;
Adenocarcinoma;
Adenocarcinoma, Mucinous;
Adenoma;
Antibodies;
Cystadenocarcinoma;
Cystadenoma;
Cytoplasm;
Epithelial Cells;
Epithelium;
Female;
Goats;
Humans;
Immunohistochemistry;
Mucins;
Ovarian Neoplasms*;
Ovary
- From:Korean Journal of Gynecologic Oncology
2005;16(2):113-122
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To evaluate the expression level, localization and distribution of 14-3-3sigma and zeta proteins in human ovarian epithelial carcinoma cells. METHODS: Epithelial carcinoma, benign epithelial tumor and normal ovarian tissues were evaluated by immunohistochemistry. For this study, mucinous adenoma (n=10), serous adenoma (n=10), mucinous adenocarcinoma (n=10) and serous adenocarcinoma (n=10) of ovary and normal ovarian tissue (n=10) were analyzed. Goat anti 14-3-3sigma polyclonal antibody and rabbit anti-14-3-3zeta polyclonal antibody were used as primary antibodies. RESULTS: The 14-3-3sigma protein expression in the glandular epithelium was not statistically different among normal ovary, cystadenoma and cystadenocarcinoma. The 14-3-3zeta protein expression in the glandular epithelium was significantly higher for cystadenocarcinoma than for cystadenoma and normal ovary. The 14-3-3sigma and zeta proteins were immunohistochemically demonstrated in the nuclei and more preferably in the cytoplasm of adenocarcinoma epithelial cells. CONCLUSION: The expression of 14-3-3zeta protein increased significantly in ovarian adenocarcinoma compared with adenoma and normal ovary. It is suggested that 14-3-3zeta protein expression may play an important role in abnormal growth of ovarian epithelial tumor cell.
