Effects of Guiqi Polysaccharide on Telomerase Activity in Premature Senescence WI-38 Cells
10.3969/j.issn.1005-5304.2015.11.014
- VernacularTitle:归芪多糖对早衰WI-38细胞端粒酶活性的影响
- Author:
Xiuying PU
;
Shuang YU
;
Wenbo FAN
;
Jing REN
;
Lu LIU
;
Xiaolong MA
;
Weijie ZHANG
- Publication Type:Journal Article
- Keywords:
Guiqi polysaccharide;
H2O2;
WI-38 cell;
senescence-associatedβ-galactosidase activity;
telomerase
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;22(11):50-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Guiqi polysaccharide (GQP) on the telomerase activity in premature senescence of WI-38 cells;To discuss its mechanism of action.Methods MTT assay was used to observe the effects of different concentrations of H2O2 on the proliferation of WI-38 cells and screened the best concentration of H2O2 to induce premature senescence cellular model in WI-38 cells. ELISA and chemical staining method were used to evaluate the protection of GQP in telomerase activity and senescence-associated β-galactosidase activity in premature cellular senescence.Results 200μmol/L H2O2 treatment could induce premature senescence in WI-38 cells. There were no significant differences in senescence-associated β-galactosidase and telomerase activity between GQP group and normal control group (P>0.05), but had significant difference with model group (P<0.01).Conclusion GQP can increase the telomerase activity and inhibit the senescence-associated β-galactosidase activity in premature senescence of WI-38 cells.