Uptake Property Study on Punicalagin in Caco-2 Cells
10.3969/j.issn.1005-5304.2015.11.022
- VernacularTitle:安石榴苷在Caco-2细胞的摄取特性研究
- Author:
Li XIE
;
Li TIAN
;
Xiaoli GAO
- Publication Type:Journal Article
- Keywords:
punicalagin;
Caco-2 cells;
uptake;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;22(11):79-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the uptake property of punicalagin in Caco-2 cells.Methods Caco-2 cell monolayer model was used to research the effects of punicalagin on cell uptake in different times, concentrations, pH values, temperatures and P-gp inhibitors. HPLC was used to determine the content of punicalagin in cells.Results The linearity of punicalagin concentration curve was found in the range of 0.024 8-0.992μg (r=0.997 9). Uptake determination of punicalagin in Caco-2 cells absorbed a certain amount of time, concentration and pH dependence and P-gp inhibitors could significantly increase the punicalagin uptake.Conclusion This method is simple and sensitive to determine the punicalagin concentration in Caco-2 cells, which is mainly passively transported. P-gp is involved in punicalagin transport process.
