Content Determination of Oleanolic Acid inStauntonia obovatifoliola Hayata subsp. urophylla by RP-HPLC

VernacularTitle:RP-HPLC测定五指那藤中齐墩果酸含量
Author: Wenjie ZHOU ; Qianping CHEN ; Youbao ZENG ; Guixiu WANG
Publication Type:Journal Article
Keywords: Stauntonia obovatifoliola Hayata subsp.urophylla; oleanolic acid; determination; RP-HPLC
From: Chinese Journal of Information on Traditional Chinese Medicine 2015;22(11):92-94
CountryChina
Language:Chinese
Abstract: Objective To establish an RP-HPLC method for content determination of oleanolic acid in Stauntonia obovatifoliola Hayata subsp.urophylla.Methods The RP-HPLC with LiChrospher C18 column (250 mm × 4.6 mm, 5μm) was used, acetonitrile-methonal-water-acetic acid-triethylamine (65:12:23:0.04:0.02) was used as mobile phase, with flow rate of 1.0 mL/min, detection wavelength at 210 nm and column temperature at 30℃.Results Oleanolic acid showed good linearity (r=0.999 9) in the range of 0.012 36-0.247 2μg;regressive equation wasY=5.13 × 105X - 6.11 × 102;the average recovery was 97.47%;RSD was 2.35% (n=6).Conclusion The method is simple, accurate and reliable, which can be used to determine the content of oleanolic acid in Stauntonia obovatifoliola Hayata subsp.urophylla.