Effects of perilla oil on the key gene expression regulating hepatic VLDL synthesis in obese rats
10.3969/j.issn.1005-4847.2015.05.006
- VernacularTitle:苏子油对肥胖大鼠肝脏极低密度脂蛋白合成关键基因表达的影响
- Author:
Xianxing XIE
;
Tao ZHANG
;
Shuang ZHAO
;
Xinglan AN
;
Lanzhi MA
;
Guangzhou ZHANG
;
Yuan LIU
- Publication Type:Journal Article
- Keywords:
n-3 polyunsaturated fatty acid;
Obesity;
animal model;
Very low density lipoproteins;
Rats
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;23(5):474-478
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to investigate the effects of high-fat diet rich in perilla oil on the expression of key genes that regulate hepatic VLDL synthesis in obese rats .Methods Sixty healthy male 5-week old SD rats were randomly divided into 2 groups.The rats in the normal control group (NC, n=12) were given normal diet, and the rats in the high fat group ( HF, n=48) were given a pure high fat diet in order to induce rat models of obesity .In the intervention period, the obesity model rats were randomly divided into 4 subgroups including consistent high fat group (CHF) and three intervention groups depending on perilla oil substitution rate of lard in CHF:20%PO, 50%PO and 100%PO.The serum triglyceride (TG) of the rats was measured after 4 weeks.Real-time PCR was applied to measure microsomal triglyceride transfer protein ( Mtp) and apolipoprotein B ( Apob) mRNA, and western blot assay was used for detecting the expression of MTP and APOB in the liver .Results Compared with the NC group , the CHF rats exhibited significantly high fat deposi-tion.The serum TG was markedly higher and the MTP and APOB were decreased at gene and protein levels in the CHF group compared with the NC group .After the intervention , PO remarkably reduced the level of serum TG and decreased he-patic fat deposition as it showed by pathological examination .At the gene and protein levels , MTP and APOB were upregu-lated by PO to different degrees .Conclusions All the three PO intervention can promote VLDL synthesis and secretion , and decrease the hepatic fat deposition in the obese rats .Furthermore , PO upregulates the expression of MTP at gene and protein levels in a dose-dependent manner .
