Simultaneous Determination of Contents of Shikonin and β,β'-dimethylacrylshikonin from Zizhu Ointment by HPLC
10.3969/j.issn.1005-5304.2015.11.021
- VernacularTitle:HPLC同时测定紫朱软膏中左旋紫草素和β,β'-二甲基丙烯酰阿卡宁含量
- Author:
Yefang DING
;
Xilin LI
;
Guobin LIU
;
Yuquan ZHANG
- Publication Type:Journal Article
- Keywords:
Zizhu Ointment;
shikonin;
β;
β'-dimethylacrylshikonin;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;22(11):76-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method to simultaneously determine the contents of shikonin andβ,β'-dimethylacrylshikonin inZizhu Ointment.Methods The contents of shikonin andβ,β'-dimethylacrylshikonin inZizhu Ointment were determined by HPLC. The chromatographic conditions were Welch Materials XB-C18 Column (4.6 mm × 250 mm, 18.5μm), with mobile phase of acetonitrile-water-formic acid (75:25:0.05), detection wavelength of 516 nm, temperature of 30℃, flow rate of 1.0 mL/min and the load of 20μL.Results Shikonin showed a good linearity in the range of 0.025 6-0.256μg (r2=0.999 5), and the average recovery rate was 98.12% (RSD=1.92%).β,β'-dimethylacrylshikonin showed a good linearity in the range of 0.174 4-2.616μg (r2=0.999 2), and the average recovery rate was 100.46% (RSD=1.17%).Conclusion This method is simple and accurate, which can be used for the quality control and evaluation of Zizhu Ointment.
