Establishment of a transgenic zebrafish line with tdTomato expression in the ultraviolet-sensitive cone photoreceptors
10.3969/j.issn.1005-4847.2015.05.003
- VernacularTitle:一种在紫外敏感型视锥细胞中特异表达 tdTomato 的转基因斑马鱼系的构建
- Author:
Zhe CHEN
;
Zhu SONG
;
Yadong WANG
;
Chengtian ZHAO
- Publication Type:Journal Article
- Keywords:
Zebrafish;
Cone photoreceptor;
Ultraviolet-sensitive;
sws1;
UV opsin;
Transgene
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;23(5):453-457
- CountryChina
- Language:Chinese
-
Abstract:
Objective To better understand the mechanisms of cone opsin transport , we set to generate a trans-genic zebrafish line with red fluorescence proteins expressing in the cone photoreceptors .Methods We used sws1 promot-er to drive the expression of a chimerical protein , in which the last 44 amino acids of rhodopsin of Xenopus laevis were fused to the C-terminus of tdTomato to restrict its localization to the outer segment of photoreceptors .Results We successfully i-solated such a transgenic zebrafish line and confirmed the localization of tdTomato by immunostaining analysis .Conclu-sions This transgenic zebrafish line will help us to better understand the transport mechanisms of cone opsins , especially the transport in live photoreceptors .
