Brazilein induced cells apoptosis in human lung cancer A549 cells and its effects on endoplasmic reticulum stress
10.3969/j.issn.1671-8348.2015.30.002
- VernacularTitle:氧化苏木素诱导肺癌 A549细胞凋亡及对内质网应激通路的影响
- Author:
Liyang TAO
;
Jianying LI
;
Jianye ZHANG
- Publication Type:Journal Article
- Keywords:
lung neoplasms;
brazilein;
A549 cells;
endoplasmic reticulum stress
- From:
Chongqing Medicine
2015;(30):4180-4182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptotic effect of brazilein on human lung cancer A549 cells and endoplasmic reticulum stress .Methods The cytotoxic activity was tested by MTT assay in A549 cells .The flow cytometry was used to detect apoptosis . Western blotting was performed to detect GRP78 and cyto c protein expression .Results The IC50 values of brazilein against A549 cells was (5 .36 ± 0 .62)μmol/L .After treatment with 0 ,5 ,10 and 20 μmol/L brazilein for 48 h ,the percent of apoptosis was (1 .15 ± 0 .32)% ,(19 .61 ± 4 .52)% ,(30 .18 ± 6 .35)% and (39 .48 ± 7 .44)% respectively .There was significant difference among the different treatment (P< 0 .05) .Compared with control group ,the protein expression of GRP78 and cytosolic cyto c was in‐creased after 5 ,10 and 20 μmol/L brazilein treated for 48 h .Conclusion Brazilein induced apoptosis in human lung cancer A549 cells though endoplasmic reticulum stress pathway .
