Dynamic changes of the expression of HIF-1,VEGF and miRNA-210 in the kidney after unilateral ureteral occlusion
10.3969/j.issn.1671-8348.2015.30.007
- VernacularTitle:肾积水后缺氧诱导因子-1、血管内皮生长因子、微小RNA-210的动态表达
- Author:
Jun DENG
;
Renrui KUANG
;
Suping CUI
- Publication Type:Journal Article
- Keywords:
hypoxia inducible factor 1;
vascular endothelial growth factor;
micro RNA 210;
unilateral ureteral occlusion
- From:
Chongqing Medicine
2015;(30):4196-4198
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of inducible factor 1(HIF‐1) ,vascular endothelial growth factor(VEGF) and miRNA‐210 and explore the regulatory mechanism after unilateral ureteral occlusion (UUO) .Methods UUO models are made by ligation unilateral ureteral with silk ,and they were divided into sham group and UUO group .All the mice were sacrificed by cer‐vical dislocation after 2 ,5 ,9 and 14 d of UUO .The kidney samples were examined for HIF‐1 mRNA ,VEGF mRNA and miRNA‐210 by quantitative real time reverse transcription polymerase chain reaction (RT‐PCR) ,and HIF‐1 protein by Western blot . Results The expression level of HIF‐1 mRNA was gradually raised after UUO (P<0 .05) ,and compared with the sham group ,the expression level of VEGF mRNA and miRNA‐210 were up regulated after 2 d of UUO and down regulated after 5 ,9 and 14 d of UUO compared with the sham group (P<0 .05);the expression level of HIF‐1 protein increased after UUO compared to the sham group .Conclusion The results of the level of HIF‐1 protein increased and VEGF mRNA and miRNA‐210 were up regulated ,which might be related to the adaptive response of kidney hypoxia and ischemia after UUO .
