Inhibitive effect of tea polyphenol on the growth of human nasopharyngeal carcinoma cell xenograft in nude mice
10.3969/j.issn.1671-8348.2015.29.017
- VernacularTitle:茶多酚对人鼻咽癌细胞裸鼠移植瘤的生长抑制作用
- Author:
Mengqiu TIAN
;
Dongjie YUAN
;
Shixing ZHENG
;
Qingyu LI
;
Shujing SHI
;
Zhiwen XU
- Publication Type:Journal Article
- Keywords:
nasopharyngeal neoplasms;
mice,nude;
tea polyphenol;
angiogenesis
- From:
Chongqing Medicine
2015;(29):4080-4082
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the inhibitive effect of tea polyphenol on the growth of human nasopharyngeal carcinoma HONE1 cell xenograft in nude mice ,and to explore the underlying mechanisms .Methods Tumor model was established by subcu‐taneous inoculation of nasopharyngeal carcinoma cell HONE1 into nude mice ,was used to evaluate the antitumor effect of tea poly‐phenol in vivo .The expression levels of VEGF were detected by real‐time PCR and western blot .Results The growth of xenograft in nude mice was significantly suppressed after application of tea polyphenol at a dose‐dependent manner .To compare with control group ,the inhibition rates were 18 .82% (P<0 .05) and 47 .66% (P<0 .05)when treated at low and high dose respectively ,With in‐creased concentration of TP ,the inhibition rates increased .Real‐time fluorescence quantitative‐PCR and western blot results showed that the expression of VEGF decreased at a dose‐dependent manner .The change of high dose group was obviously ,the difference was statistically significant(P<0 .05) .Conclusion Tea polyphenol could significantly inhibit the growth of human nasopharyngeal carcinoma HONE1 cell xenograft in nude mice ,probably by down regulating the VEGF protein level to inhibit tumor angiogenesis effects .
