Hepatic bile acids and the expression of FXR,SHP,and Cyp7α1 in normal rats during pregnancy and lactation
10.3969/j.issn.1671-8348.2015.30.004
- VernacularTitle:孕期和哺乳期大鼠肝脏胆酸和胆酸调控法尼醇X受体、小异二聚体伴侣受体和胆固醇7α-羟化酶的变化
- Author:
Hongmei XIE
;
Dan ZHANG
;
Jie LIU
- Publication Type:Journal Article
- Keywords:
pregeant rats;
postnatal rats;
liver bile acids;
nuclear receptor farnesoid X;
small different dimer partner;
choles-terol 7αhydroxylase
- From:
Chongqing Medicine
2015;(30):4187-4189
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the accumulation of liver bile acids and bile acid regulated nuclear receptor farnesoid X (FXR) and its target gene cholesterol 7α hydroxylase (Cyp7α1) and small different dimer partner (SHP) in normal rats during pregnancy and lactation .Methods SD rats were maintained in the SPF‐grade facilities for time‐controlled mate .Livers were collect‐ed at Gestation Day (GD) 10 ,14 ,and 19 and Postnatal Day (PND) 1 ,7 ,14 and 21 d .Hepatic bile acids were quantified with a total bile acid assay kit ,and mRNA expression of FXR ,Cyp7α1 and SHP was determined by RT‐PCR .Western blot was used for Cyp7α1 protein quantification .Results In livers of normal rats ,bile acids was not elevated during pregnancy but was increased during lacta‐tion .The bile acid synthesis rate limiting enzyme Cyp7α1 increased during lactation but were normal during pregnancy .SHP was 6 fold higher at the late pregnancy ,and slightly higher during lactation .FXR was increased at postpartum .Conclusion Under normal circumstances ,hepatic bile acid synthesis during pregnancy was controlled by SHP feedback ,while during lactation ,hepatic bile acids increased ,along with increased Cyp7α1 and FXR .
