The expression of P16,Ki-67 in cervical squamous epithelial lesions and the significance of their detection combined with HPV
10.3969/j.issn.1673-4130.2015.20.009
- VernacularTitle:P16、Ki-67在鳞状上皮病变宫颈组织中的表达及其与 HPV 联合检测的意义
- Author:
Yanzhen PAN
;
Hongmei DING
- Publication Type:Journal Article
- Keywords:
cervical intraepithelial neoplasia;
cervical cancer;
gene,Ki-67;
gene,P16;
human papillomavirus
- From:
International Journal of Laboratory Medicine
2015;(20):2942-2944
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of P16 ,Ki‐67 in cervical squamous epithelial lesions and the significance of their detection combined with human papillomavirus(HPV) .Methods 188 cases of cervical tissues from surgical excision were collected , including 48 cases of cervical cancer ,100 cases of cervical intraepithelial neoplasia(CIN) ,and 40 cases of chronic cervicitis ,which were set as cervical cancer group ,CIN group and cervicitis group ,respectively .Expression of P16 ,Ki‐67 in cervical tissue were de‐tected by using immunohistochemistry ,and the HPV infection were detected by using PCR technology .Results The positive ex‐pression rates of P16 and Ki‐67 in cervical cancer ,CIN group was significantly higher than those in cervicitis group(P<0 .05) ,and the positive expression rates of P16 and Ki‐67 in cervical cancer group were higher than those in CINⅠ and CINⅡ group (P<0 . 05) ,but between the cervical cancer group and CIN Ⅲ group there were no statistically significant difference(P>0 .05) .With the increase of CIN level ,the positive expression rates of P16 and Ki‐67 increased ,among the three CIN groups there were significant difference(P<0 .05) .In cervical cancer group and CINⅢ group ,the expression rates of P16 and Ki‐67 were both significantly high‐er than the other 3 groups(P<0 .05) .In CINⅠ - Ⅲ groups ,the over expression rates of P16 and Ki‐67 were statistically different (P<0 .05) .The expression of P16 positively correlated with the positive rates of HPV infection (rs =0 .706 ,P=0 .011);the ex‐pression of Ki‐67 positively correlated with the positive rates of HPV infection(rs=0 .695 ,P=0 .021) .Conclusion CIN and cervi‐cal cancer of early stage could be diagnosed and the pathological progress of CIN could be predicted by using the combined detection of P16 ,Ki‐67 and HPV .
