Development and characterization analysis of a new type of electrochemical immunosensor for the detection of ochratoxin A
10.3969/j.issn.1673-4130.2015.20.012
- VernacularTitle:一种用于检测赭曲霉毒素 A 的新型电化学免疫传感器的构建及表征分析
- Author:
Xian ZHANG
;
Xianxian YANG
;
Ying QING
;
Jingfu QIU
;
Chaorui LI
- Publication Type:Journal Article
- Keywords:
electrochemical immunosensor;
ochratoxin A;
single-walled carbon nanotubes;
chitosan
- From:
International Journal of Laboratory Medicine
2015;(20):2950-2952
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new type of electrochemical immunosensor for the detection of ochratoxin A (OTA ) . Methods Double layers of self‐assembly immunosensor for the detection of OTA were constructed based on the composite single‐walled carbon nanotubes(SWNTs)/chitosan(CS) membrane immobilized on glassy carbon electrode(GC) .Scanning electron mi‐croscopy(SEM) ,square wave voltammetry and cyclic voltammetry were used to analyze the characterization of the sensor ,then its specificity for detection was studied .Results SWNTs/CS composit membrane could increase the sensitivity of OTA detection sig‐nificantly ,and effectively distinguish the different types of mycotoxins .Conclusion The electrochemical immunosensor developed in the study is easy to operate and could detect OTA rapidly with good specificity and low detection limit .
