Silencing Nestin expression mediated by lentivirus inhibits human esophageal cancer ECA 109 cell proliferation
- VernacularTitle:慢病毒介导的Nestin基因沉默抑制人食管癌ECA109细胞增殖
- Author:
Yanni CAO
;
Huachuan WANG
;
Congyi WANG
;
Shenyin ZHU
;
Jianhu WEN
- Publication Type:Journal Article
- Keywords:
Nestin;
esophageal;
proliferation;
gene silencing
- From:
Basic & Clinical Medicine
2015;(9):1214-1218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Nestin gene silencing on the proliferation of human esophageal cancer ECA109 cells and possible mechanism .Methods Lenti-Nestin was constructed and transfected into ECA109 cells to establish a stable Nestin-silencing cell line Lenti-Nestin.Blank group , scrambled group , Lenti-Nestin group were set up .The expressions of Nestin , c-myc and cyclin D1 mRNA and protein levels were detected by qRT-PCR and Western blot .The cell proliferation was analyzed by CCK 8 assay .Results The stable Nestin-si-lencing cell line was successfully established .The expression of Nestin mRNA ( P<0.01 ) and protein ( P<0.05 ) levels were reduced significantly and the downregulation evidently suppressed cell proliferation ( P<0.01 ) and col-ony forming capacity ( P<0.05 ) compare with the scrambled group and blank group .However ,The c-myc and cy-clin D1 expression levels in ECA 109 cells in Lenti-Nestin group were significantly lower than that of scrambled group and blank group ( P<0.05 ) .Conclusions Knockdown Nestin expression significantly inhibits the level of esophageal cancer ECA109 cells proliferation,which may act via influencing the expression of c-myc, cyclin D1.
