Role of NF-κB in sevoflurane-induced release of inflammatory factors in mouse microglias
10.3760/cma.j.issn.0254-1416.2015.07.009
- VernacularTitle:NF-κB在七氟醚诱发小鼠小胶质细胞释放炎性因子中的作用
- Author:
Min SUN
;
Jihui YIN
;
Yi LIU
;
Zuolei CHEN
;
Ruyi ZHANG
- Publication Type:Journal Article
- Keywords:
NF-kappa B;
Anesthetics,inhalation;
Microglia;
Cytokines
- From:
Chinese Journal of Anesthesiology
2015;35(7):808-810
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of nuclear factor kappa B (NF-κB) in sevofluraneinduced release of inflammatory factors in mouse microglias.Methods Microglial cells obtained from newborn C57BL/6 mice (aged 2-3 days) were seeded in 24-well plates (density 1 × 105 cells/ml, 1 ml/well) , a total of 80 wells.The cells were randomly divided into 4 groups using a random number table (n =20 each) : control group (group C);sevoflurane group (group S);NF-κB selective inhibitor pyrrolidine dithiocarbamate (PDTC) group (group P);PDTC + sevoflurane group (group P +S).In S and P+S groups, 4.1％ sevoflurane was inhaled for 6 h.In P+S group, 10 μmol/L PDTC was added at 1 h before sevoflurane inhalation.At 6 h of incubation with sevoflurane, NF-κB activity and expression and levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were determined.Results Compared with group C, the NF-κB activity and levels of IL-6 and TNF-α were significantly increased in group S, and no significant change in the above parameters was found in the other two groups.Compared with group S, the NF-κB activity and levels of IL-6 and TNF-α were significantly decreased in group P+S.There was no significant difference in NF-κB expression between the four groups.Conclusion NF-κB mediates sevoflurane-induced release of inflammatory factors in mouse microglias.
