The study on the expression of XRCC1 in chronic myelocytic leukemia
10.3969/j.issn.1673-4130.2015.20.035
- VernacularTitle:X RCC1基因在白血病中表达的研究
- Author:
Xiaojing CHAI
;
Juan LI
;
Li LU
- Publication Type:Journal Article
- Keywords:
X-ray cross complementing group 1;
leukemia;
gene polymorphism;
methylation
- From:
International Journal of Laboratory Medicine
2015;(20):3005-3006
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether XRCC1 was associated with the occurrence of leukemia through the comparison of XRCC1 gene′s polymorphism and hypermethylation between leukemia patients and healthy people .Methods Restriction fragment length polymorphism polymerase chain reaction (PCR‐RFLP) and methylation specific polymerase chain reaction(MSP) were used to detect the polymorphism and promoter region′s methylation of XRCC1 gene in 150 patients with leukemia(patients group) and 150 healthy persons (control group) .Results In different types of leukemia patients ,the genotype of XRCC1 gene loci rs1799782 , rs25487 and rs25489 loci changed in different degrees .The positive rate of XRCC1 gene methylation in different subgroups of pa‐tients group were not statistically significantly different from that of control group(P>0 .05) .Conclusion Genotype distribution and allele frequency and leukemia susceptible have some correlation ,but hypermethylation phenomenon may not exist in XRCC1 gene in leukemia .
