Mutations in the 23S rRNA Gene of Helicobacter pylori Associated with Clarithromycin Resistance.
10.3346/jkms.2002.17.5.599
- Author:
Kyung Suk KIM
1
;
Jung Oak KANG
;
Chang Soo EUN
;
Dong Soo HAN
;
Tae Yeal CHOI
Author Information
1. Department of Clinical Pathology, Major Woman's Hospital, Seoul, Korea. jokang@hanyang.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Helicobacter pylori;
Clarithromycin;
Resistance;
23S rRNA Gene;
Mutation
- MeSH:
Amino Acid Sequence;
Anti-Bacterial Agents/*pharmacology;
Base Sequence;
Clarithromycin/*pharmacology;
DNA, Bacterial/genetics;
Drug Resistance, Bacterial/genetics;
*Genes, Bacterial;
Helicobacter Infections/drug therapy/microbiology;
Helicobacter pylori/*drug effects/*genetics;
Humans;
Korea;
Molecular Sequence Data;
Mutation;
RNA, Bacterial/genetics;
RNA, Ribosomal, 23S/genetics;
Sequence Homology, Nucleic Acid
- From:Journal of Korean Medical Science
2002;17(5):599-603
- CountryRepublic of Korea
- Language:English
-
Abstract:
Among 12 clarithromycin-resistant Helicobacter pylori strains isolated in Guri, Korea, 8 showed an adenine to guanine mutation at position 2143 (formerly A2144G or E. coli 2059) in the 23S rRNA gene by the PCR-restriction fragment length polymorphism (RFLP) method. The remaining 4 strains, digested by neither BsaI nor BbsI, showed a thymine to cytosine mutation at position 2182 (T2182C) by direct sequencing of the PCR products. The T2182C mutants showed a tendency of higher levels of minimum inhibitory concentration to clarithromycin than the A2143G mutants. In conclusion, either the A2143G or the T2182C mutation was present in 100% of clarithromycin-resistant H. pylori isolates examined. The PCR-RFLP technique with restriction enzymes BbsI and BsaI was a rapid and relatively simple method to detect the clarithromycin resistance. But undigested isolates were quite frequent among our isolates (33.3%), the PCR-RFLP method with restriction enzymes BbsI and BsaI should not be used alone, and development of other rapid detection method for clarithromycin resistance is mandatory.
