Mass spectrometry assessment of the protective effects of Zhizi Dahuang decoction on alcoholic liver injury

VernacularTitle:栀子大黄汤对酒精性肝损伤保护作用的质谱评价
Author: Xinxin LI ; Linfei CHEN ; Yongzhong OUYANG ; Huanwen CHEN ; Fang FENG
Publication Type:Journal Article
Keywords: Zhizi Dahuang decoction; alcoholic liver injury; principal component analysis; mass spectrometry imaging
From: Journal of China Pharmaceutical University 2015;(5):579-586
CountryChina
Language:Chinese
Abstract: Liquid-assisted surface desorption atmospheric pressure chemical ionization mass spectrometry (LA-DAPCI-MS)was used directly for the analysis of healthy rat liver (normal control group);acute alcoholic injury rat liver(alcohol model group)and Zhizi Dahuang decoction-treated rat liver (ZZDHD group)sections with the mass spectrometry images obtained simultaneously.Principal component analysis (PCA)was employed for the differentiation of livers of the groups;and 3 phospholipids;PC (34 ∶2);PC(36 ∶4)and PC(38 ∶4);were found to be the main factors.LA-DAPCI mass spectrometry images showed that the 3 phospholipids were evenly distrib-uted in liver under the spatial resolution of 0.01 mm2.Contents of 3 PCs in the model group were decreased in alcohol model group compared to the normal control group and the ZZDHD group;which revealed the relationship between acute alcoholic liver injury and intrahepatic phospholipid variation.The results showed that ZZDHD had protective effect on acute alcoholic liver injury-induced intrahepatic phospholipid variations at the molecular level.