Mass spectrometry assessment of the protective effects of Zhizi Dahuang decoction on alcoholic liver injury
10.11665/j.issn.1000-5048.20150511
- VernacularTitle:栀子大黄汤对酒精性肝损伤保护作用的质谱评价
- Author:
Xinxin LI
;
Linfei CHEN
;
Yongzhong OUYANG
;
Huanwen CHEN
;
Fang FENG
- Publication Type:Journal Article
- Keywords:
Zhizi Dahuang decoction;
alcoholic liver injury;
principal component analysis;
mass spectrometry imaging
- From:
Journal of China Pharmaceutical University
2015;(5):579-586
- CountryChina
- Language:Chinese
-
Abstract:
Liquid-assisted surface desorption atmospheric pressure chemical ionization mass spectrometry (LA-DAPCI-MS)was used directly for the analysis of healthy rat liver (normal control group);acute alcoholic injury rat liver(alcohol model group)and Zhizi Dahuang decoction-treated rat liver (ZZDHD group)sections with the mass spectrometry images obtained simultaneously.Principal component analysis (PCA)was employed for the differentiation of livers of the groups;and 3 phospholipids;PC (34 ∶2);PC(36 ∶4)and PC(38 ∶4);were found to be the main factors.LA-DAPCI mass spectrometry images showed that the 3 phospholipids were evenly distrib-uted in liver under the spatial resolution of 0.01 mm2.Contents of 3 PCs in the model group were decreased in alcohol model group compared to the normal control group and the ZZDHD group;which revealed the relationship between acute alcoholic liver injury and intrahepatic phospholipid variation.The results showed that ZZDHD had protective effect on acute alcoholic liver injury-induced intrahepatic phospholipid variations at the molecular level.