Research advances of recombinant coagulation factor VII expression and synthesizing mechanism
10.11665/j.issn.1000-5048.20150518
- VernacularTitle:重组凝血因子Ⅶ表达和合成机制的研究进展
- Author:
Lin PENG
;
Xiao YU
;
Yanfei CAI
;
Jian JIN
;
Huazhong LI
- Publication Type:Journal Article
- Keywords:
recombinant coagulation factor VII;
hemophilia;
hemostasis mechanism;
mammalian cell;
post-transla-tional modification
- From:
Journal of China Pharmaceutical University
2015;(5):623-628
- CountryChina
- Language:Chinese
-
Abstract:
Haemophilia is caused by lack of coagulation factor VIII or IX in patients′blood with inadequate hemostasis.Currently recombinant coagulation factor VII(rFVII)produced in different cells is used against clini-cal bleeding of haemophilia patients.To enhance the production and activity of rFVII;some eukaryotic cells such as baby hamster kidney(BHK);Chinese hamster ovary(CHO);insect cell and fish embryo;were used to express rFVII.Meanwhile;the effect of functional gene on the activity of rFVII and the limitation of rFVII production caused by post-translational modification were investigated by different methods.The role of rFVII in hemostasis;synthesis of rFVII in different eukaryotic cells and impact on production of post-translational modification are reviewed in this article.
