Research advances in quantitative detection of single cell protein
10.11665/j.issn.1000-5048.20150502
- VernacularTitle:单细胞蛋白定量检测方法研究进展
- Author:
Yiyun SHEN
;
Xiemin QI
;
Qinxin SONG
;
Guohua ZHOU
- Publication Type:Journal Article
- Keywords:
single cell protein;
quantitative detection;
research progress;
micr of luidics;
microwell-based technol-ogy;
optical fiber nanobiosensor;
probe technology;
mass spectrometry
- From:
Journal of China Pharmaceutical University
2015;(5):521-531
- CountryChina
- Language:Chinese
-
Abstract:
Proteins presence and differences of the expression level can clarify the physiological or pathological changes in organisms;so the quantitative detection of proteins is vital for disease mechanism research;diagnosis and prognosis evaluation.Traditional protein quantitation methods at the tissue level reflected the average protein expression in cells;but ignore the differences between individual cells.In contrast;approaches for quantitative detection at single-cell level can better reflect the differences.Recently;a number of approaches for such detec-tion have been proposed;including microfluidics;microwell-based technology;optical fiber nanobiosensor;activity-based probe technology and mass spectrometry.The principles;advantages and drawbacks of these approaches are briefly introduced in this review.
