Expression of miRNA-1247-3P in acute respiratory distress syndrome cell model and its function

VernacularTitle:MiRNA-1247-3P在急性呼吸窘迫综合征细胞模型中的表达及功能分析
Author: Dongliang CHENG ; Yuan LIANG ; Yanchen CHEN ; Di QING ; Changsong SHI
Publication Type:Journal Article
Keywords: Acute respiratory distress syndrome; miR-1247-3P; Surfactant protein A; A549 cell
From: The Journal of Practical Medicine 2015;(17):2802-2804
CountryChina
Language:Chinese
Abstract: Objective To detect the expression pattern of microRNA in A549 cells treated by lipopolysaccharide, study the expression of miRNA-1247-3P in A549 cells under LPS treatment and explore the possible mechanism of miRNA-1247-3P in A549 cells under LPS treatment. Methods A549 cells were divided into experimental and control groups. Immunocytochemical method and RT-PCR were used to detect the changes of SP-A and SP-C. The expression of miRNAs were detected using miRNAs array in different groups. The key miR-1247-3P was collected to detect the changes of miR-1247-3P in all groups using quantitative real-time polymerase chain reaction. Results Compared with control group, the expressions of SP-A and SP-C were significantly decreased in the experimental groups (P < 0.05). MiRNA array showed that 31 miRNAs were up-regulated and 3 miRNAs were down-regulated. Compared with control group, the expression of miR-1247-3P was significantly increased in the experimental groups (P < 0.05). Conclusion The increased expression of miR-1247-3P may play an important role in the pathogenesis of ARDS.