Determination of Hexabromocyclododecanes Enantiomers in Earthworm and Soil by Chiral High Performance Liquid Chromatography-Isotopic Dilution-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.150244
- VernacularTitle:手性高效液相色谱-同位素稀释串联质谱法测定土壤及蚯蚓中的六溴环十二烷对映体
- Author:
Qin TIAN
;
Ling TONG
;
Shuling SONG
;
Xiaochun WANG
;
Xingchun JIAO
;
Runfei JIANG
- Publication Type:Journal Article
- Keywords:
Isotope dilution;
High performance liquid chromatography-tandem mass spectrometry;
Hexabromocyclododecanes;
Enantiomer;
Soil;
Earthworm
- From:
Chinese Journal of Analytical Chemistry
2015;(9):1383-1388
- CountryChina
- Language:Chinese
-
Abstract:
An enantioselective method was developed for the separation and determination of three chiral hexabromocyclododecanes ( HBCDs ) including α-HBCD, β-HBCD, γ-HBCD in soil and earthworm by HPLC-ID-MS/MS. d18-HBCDs used as internal standards were added to the samples before extraction. HBCDs enantiomers were extracted from soil by accelerated solvent extraction ( ASE ) with n-hexane/DCM (1:1,V/V) at 100℃ and 10 MPa for 5 min, and further cleaned up using silica column. HBCDs enantiomers were extracted from earthworm by vortex turbulence with ethyl acetate. The extracts were orderly sulphonated by sulfuric acid, and purified by silica column. For all HBCDs enantiomers, good linearities were obtained in the concentration range of 0. 25-50 ng/mL. Limits of detection ( LOD) and limits of quantification ( LOQ) were 0. 00544-0. 00766 ng/g and 0. 0173-0. 0244 ng/g, respectively in soil. The recoveries of spiked samples at 0. 05 and 2. 5 ng/g levels were 80. 0%-95. 9% with relative standard deviations ( RSD, %) of 5. 7%-11. 9% in soil. Limits of detection (LOD) and limits of quantification (LOQ) were 0. 0103-0. 0148 ng/g and 0. 0328-0. 0471 ng/g, respectively in earthworm. The recoveries of spiked samples at 0. 1 and 5 ng/g levels were 78. 0% -94. 4% with relative standard deviations ( RSD, %) of 6. 1% -12. 2% in earthworm. This method can meet the requirements of determination of trace HBCDs in soil and earthworm.
