Preventive effect of Polygonum Multiflorum on deteriorated micro-structure and biomechanical properties induced by prednisone
10.3969/j.issn.1001-1978.2015.09.019
- VernacularTitle:何首乌对泼尼松致大鼠股骨微结构及生物力学改变的预防作用
- Author:
Manru ZHOU
;
Jin LI
;
Jingkai WU
;
Xiaobin ZENG
;
Jingfeng CHEN
;
Liao CUI
;
Yuyu LIU
- Publication Type:Journal Article
- Keywords:
Polygonum Multiflorum;
prednisone;
bone micro-structure;
bone biomechanics;
osteoporo-sis;
rats
- From:
Chinese Pharmacological Bulletin
2015;(9):1273-1279
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the preventive effect of Polygonum Multiflorum (PM)on the deteriorated mi-cro-structure and biomechanical properties induced by prednisone.Methods Ninety 6-month-old male Sprague-Dawley rats were randomly divided into nine groups,which were control,prednisone,CAL,30%ethanol eluent of the PM(H,M,L),PM(H,M,L). Prednisone was gavaged to rat for 21 weeks as model group of osteoporosis.Meanwhile,tested herbal ab-stract were orally administrated to the modeled rats in-duced by prednisone.At the end of the experiment, the right femur was collected for micro-CT scanning, three-dimensional reconstruction and biomechanical test.Results Compared with the control group,mod-el group showed destruction of bone microarchitecture, BV /TV fell 28.6%(P <0.05),bone biomechanical parameters decreases,and stiffness fell 29.7%(P <0.01 ). Compared with the model group, positive group had significantly improved effect on bone micro-architecture,and biomechanical parameters,BV /TV increased 46.7%(P <0.01 ),and stiffness increased 25.9%(P <0.01 ).30% ethanol eluent of the PM (M,L)dose may improve bone microstructure by in-creasing BV /TV 46.7% (P <0.01 ),40.0% (P <0.05)respectively,PM(H)may improve the biome-chanical parameters by increasing stiffness 24.7%(P<0.05),and 30% ethanol eluent of the PM(H)and PMhigh-dose may improve the biomechanical parame-ters,but not as positive group.Conclusions Predni-sone reduces biomechanical properties of rat femur and deteriorates femoral microstructure.30% ethanol eluent of the PM(M,L)and PM(H)plays a preventive role in the changes of micro-structural and biomechanical properties by prednisone,and increases BMD,whereas other groups have no significant preventive effect.
