Role of PCSK9 and IDOL in curcumin accelerating LDL-C uptake in HepG2 cells
10.3969/j.issn.1001-1978.2015.09.021
- VernacularTitle:PCSK9及 IDOL 在姜黄素促进 HepG2细胞摄取 LDL-C 中的作用
- Author:
Lu OU
;
Caiping ZHANG
;
Ying LIU
;
Xinhui QIAO
;
Yanni MA
;
Chun OU
;
Xiaobo HU
;
Yin TIAN
;
Shiyin LONG
- Publication Type:Journal Article
- From:
Chinese Pharmacological Bulletin
2015;(9):1286-1291
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the lipid-lowering mecha-nisms of curcumin from the molecular levels and pro-vide scientific basis for clinical development of lipid-lowering drugs.Methods Using oil red O staining and enzymic to determinate the levels of cholesterol in HepG2 cells.Moreover,uptaking of DiI-LDL was also measured.The expressions of mRNA and protein were detected by RT-Q-PCR and Western blot.Results The red lipid droplets and the levels of TC and FC sig-nificantly increased in HepG2 cells after treated with curcumin.The orange red fluorescence was higher than that of control.Curcumin could promote the expression levels of mRNA and protein of SREBP2 and LDLR, what′s more,curcumin could reduce the expression of the mature PCSK9 level and IDOL protein.Conclu-sion Curcumin accelerates LDL-C uptake probably via downregulating the expression of PCSK9 and IDOL in HepG2 cells.
