Effect of Isoliquiritigenin on C6 glioma cell proliferation and differentiation
10.3969/j.issn.1001-1978.2015.09.023
- VernacularTitle:异甘草素对大鼠 C6胶质瘤细胞增殖和分化的影响
- Author:
Yajuan LI
;
Lu GAN
;
Zhanyang WANG
;
Lihong QIU
;
Yingying SI
;
Hong ZHANG
;
Chengjun MA
;
Ji LI
;
Xiling SUN
;
Zhenhua WANG
- Publication Type:Journal Article
- Keywords:
Isoliquiritigenin;
C6 glioma cell;
prolifera-tion;
differentiation;
colony formation;
giemsa staining;
astrocyte;
GFAP
- From:
Chinese Pharmacological Bulletin
2015;(9):1298-1303
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of isoliquiri-tigenin(ISL)on C6 glioma cell proliferation and differ-entiation.Methods C6 glioma cells’viability and proliferation were respectively measured by SRB test. Colony formation of C6 glioma cells from different groups was assayed.After culturing the cells from each group,giemsa staining was used to observe cell mor-phology.RT-PCR was applied to detect mRNA expres-sion of GFAP.Western blot was applied to detect the expression of GFAP.Results ISL effectively inhibited the viability of C6 glioma cells when compared with the control group in a concentration-dependent manner (P<0.01).The morphological observation under light mi-croscope showed that:in the control group,most of the undifferentiated C6 cells showed long fusiform and po-lygonal shape.Compared to the control group,the C6 cells treated with ISL revealed alteration in morphology such as astrocytes with smaller smooth,round body and much finer longer,tapering processes.The cloning for-mation rate detection revealed that:the colonies in the control group semerged earlier and were larger than those experimental ones,the cloning formation rate was higher,while almost no effective cells colony emerged in ISL treated groups(P <0.01 ).Western blot and RT-PCR analysis showed that GFAP expression in the ex-perimental groups increased(P <0.01).Conclusion ISL may inhibit the proliferation of C6 glioma cells and induce their differentiation.
