Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide
10.11895/j.issn.0253-3820.150327
- VernacularTitle:硒-汞双元素标记策略识别硒蛋白/多肽
- Author:
Ming XU
;
Limin YANG
;
Qiuquan WANG
- Publication Type:Journal Article
- Keywords:
Selenium;
Mercury;
Selenoprotein selenopeptide;
Inductively coupled plasma mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2015;(9):1265-1271
- CountryChina
- Language:Chinese
-
Abstract:
An endogenous element-label plus exogenous element-tag strategy was proposed for inductively coupled plasma mass spectrometry ( ICP-MS) to screen and discriminate a family of ultratrace but biological important biomolecules. The feasibility of this novel idea has been demonstrated when setting seleno ( SeCys) and Se-containing ( SeMet) proteins ( peptides) as an example. Se-label naturally occurring in the biomole-cules acts an identifier for picking them up out of large amount of various coexisting proteins ( peptides) , and CH3 Hg-tag that can bind to SeCys but not SeMet fulfills the task of discriminating seleno and Se-containing ones based on the Se and Hg signals on ICP-MS. After confirmed using SeCys and GPx1, the Se-Hg dual-element labeling strategy together with ICP-MS was applied to screen and discriminate seleno and Se-contai-ning proteins ( peptides) in the water-soluble extracts of Se-enriched yeast, and seven selenoproteins ( pep-tides) were detected with both 202 Hg and 82 Se signals out of fifteen Se-containing species using RPLC/ICP-MS, providing valuable information for further identification using a high-resolution structure-selective mass spectrometer. This endogenous element-label plus exogenous element-tag dual-element approach implies that ICP-MS is not only able to quantify targeted proteins ( peptides) but also helpful to recognize targeted proteins during a discovery-based proteomic study.
