Fabrication of IgG Functionalized Mn Doped ZnS Quantum Dots for Low Background Phosphorescent Sensing of IgG in Human Serum
10.11895/j.issn.0253-3820.150352
- VernacularTitle:功能化Mn掺杂ZnS量子点用于人血清中IgG的低背景磷光检测
- Author:
Baoyan ZHU
;
Chengxiong YANG
;
Xiuping YAN
- Publication Type:Journal Article
- Keywords:
Manganese doped zinc sulfide quantum dots;
Immunoglobulin G;
Gold nanoparticals;
Phosphorescence
- From:
Chinese Journal of Analytical Chemistry
2015;(9):1272-1277
- CountryChina
- Language:Chinese
-
Abstract:
IgG functionalized Mn doped ZnS quantum dots ( IgG-QDs ) were fabricated via the amide condensation between the carboxy groups on the surface of Mn doped ZnS quantum dots and the amino groups on the surface of IgG. The IgG antibody functionalized Au nanoparticals were synthesized via the electrostatic interaction between the Au nanoparticals and IgG antibody. Based on the fluorescence resonance energy transfer effect between the IgG-QDs and the IgG antibody functionalized Au nanoparticals, the IgG-QDs could be applied for low background phosphorescent sensing of IgG with the linear range of 1-10 μg/mL and the limit of detection of 0. 45 μg/mL. The proposed method was successfully applied to detect IgG in human serum without the disturbance of the background fluorescence and scattered light.
