Determination of Selenium Species in Burdock and Panax Notoginseng Using Ultrasonic Assistant Extraction Combined with High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry
10.11895/j.issn.0253-3820.150574
- VernacularTitle:超声辅助提取结合高效液相色谱-电感耦合等离子体质谱联用技术测定牛蒡和三七中硒形态
- Author:
Yupin CAO
;
Lizhen YAN
;
Hongli HUANG
;
Biyang DENG
- Publication Type:Journal Article
- Keywords:
Selenium;
High performance liquid chromatography-inductively coupled plasma mass spectrometry;
Burdock;
Panax notoginseng;
Ultrasonic extraction
- From:
Chinese Journal of Analytical Chemistry
2015;(9):1329-1334
- CountryChina
- Language:Chinese
-
Abstract:
A high performance liquid chromatography-inductively coupled plasma mass spectrometric ( HPLC-ICP-MS) method for analysis of selenocystine (SeCys2), selenite (SeⅣ), selenomethionine (SeMet) and selenate ( SeⅥ) was described. Chromatographic separation was performed using 8 mmol/L citric acid solution (pH 5). The potentially interfering 40Ar40Ar+ at selenium masses of m/z 80 was reduced using CH4 as a reactive cell gas in the dynamic reaction cell. The selenium species was extracted using ultrasonic extraction system with a mixture of protease K and lipase. The limits of detection for SeCys2 , SeⅣ, SeMet, and SeⅥwere observed as 0. 088, 0. 033, 0. 30, and 0. 17 ng/mL, respectively. The correlation coefficients were greater than 0 . 9995 . The recoveries were observed in the range of 95 . 1%-114 . 6%. The developed method was successfully applied for the determination of selenium species in Burdock and Panax notoginseng.
