Femtosecond laser promotes the proliferation and differentiation of human gingival fibroblasts on pure titanium surface
10.3969/j.issn.2095-4344.2015.34.004
- VernacularTitle:飞秒激光处理促进人牙周成纤维细胞在纯钛材料表面的增殖和分化
- Author:
Ju LI
;
Xiuhua DONG
- Publication Type:Journal Article
- Keywords:
Titanium;
Lasers;
Surface Properties;
Fibroblasts
- From:
Chinese Journal of Tissue Engineering Research
2015;(34):5428-5432
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Currently, the implant surface is optimized to promote the osseointegration of artificial dental implants, which has become a research focus in recent years. But the femtosecond laser is less reported to modify the titanium surface so as to promote fibroblast proliferation and differentiation. OBJECTIVE: To observe the effect of femtosecond laser on the proliferation and differentiation of human periodontal ligament fibroblasts on titanium surface. METHODS: Human periodontal ligament fibroblasts were respectively inoculated on different titanium surfaces of the three groups (femtosecond laser group, TiO2 particle blasted group and pure titanium group). Then differentiation and proliferation of human periodontal ligament fibroblasts were observed. RESULTS AND CONCLUSION:There was a significant difference in the surface roughness among these three groups, and compared with the pure titanium group, the attached cels were more in the femtosecond laser group and TiO2 particle blasted group. mRNA expressions of RUNX2 and OSX were highest in the femtosecond laser group folowed by TiO2 particle blasted group and pure titanium group in order. These findings indicate that pure titanium treated with femtosecond laser has fine biological property and may be more feasible for human periodontal ligament fibroblasts to attach, grow and proliferate.
