Protective effects of resveratrol on sepsis and its involved mechanisms
10.3969/j.issn.1001-1978.2015.09.008
- VernacularTitle:白藜芦醇对于小鼠败血症休克的保护作用及其作用机制的研究
- Author:
Hongying CHEN
;
Yajing JI
;
Dan WU
;
Yao WU
;
Wei JIANG
- Publication Type:Journal Article
- Keywords:
sepsis;
LPS;
resveratrol;
SIRTs;
NF-κB;
TNF-α
- From:
Chinese Pharmacological Bulletin
2015;(9):1216-1221
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of resveratrol (Res)on septic mice and LPS-insulted H9c2 cells,as well as its involved mechanisms.Methods By use of a mouse cecal ligation and puncture-induced septic model (CLP),the survival of septic mice was evalua-ted after resveratrol treatments.H9c2 cells were insul-ted by LPS and then treated with resveratrol,the mR-NA expressions of TNF-α,SIRT1 and other class III HDAC members were detected using RT-PCR and real-time PCR,Finally,the protein levels of nuclear p65, an important subunit of NF-κB,were measured in H9c2 cells using Western blot assay,to reveal the effect of resveratrol on LPS-induced nuclear transloca-tion of NF-κB.Results Compared with the control septic animals,intraperitoneal injection of resveratrol (1 or 5 mg·kg -1 ·d -1 )significantly increased the survival of septic mice.Furthermore,resveratrol signif-icantly increased mRNA expressions of SIRT1,SIRT2, SIRT6 and SIRT7 in LPS-insulted H9c2 cells.Res-veratrol also remarkably inhibited LPS-induced nuclear translocation of NF-κB.Conclusion An appropriate dose of resveratrol protects septic mouse hearts from the injury induced by LPS through the activation of SIRT family members and the inhibition of NF-κB pathway.
