An Approach to Diagnosing Gastrointestinal Stromal Tumors Using Immunohistochemistry of c-kit and PDGFRA with Molecular Analysis.
- Author:
Jeong Shik KIM
1
;
Jae Hoon KIM
;
Hyun Jin OH
;
In Soo SUH
;
Jong Gwang KIM
;
Byung Wook KANG
;
Wan Sik YU
;
Ho Young CHUNG
;
Han Ik BAE
Author Information
1. Department of Pathology, Kyungpook National University Hospital, Kyungpook National University School of Medicine, Daegu, Korea. baehi@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Gastrointestinal stromal tumors;
c-kit;
Receptors, platelet-derived growth factor alpha (PDGFRA);
Mutation;
Sequence analysis
- MeSH:
DNA;
Exons;
Gastrointestinal Stromal Tumors;
Gastrointestinal Tract;
Immunohistochemistry;
Paraffin;
Protein Kinases;
Receptors, Platelet-Derived Growth Factor;
Sequence Analysis
- From:Korean Journal of Pathology
2010;44(2):173-178
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumors in the gastrointestinal tract. Recently, many methods for the diagnosis of GIST have been developed including molecular diagnosis. METHODS: We selected 90 cases of GIST that had presented at Kyungpook National University Hospital between 1998 and 2007. Tissue microarrays were made using core areas of tumor tissues. Immunohistochemical staining for c-kit, protein kinase C-theta, and platelet-derived growth factor receptor alpha (PDGFRA) was done. Direct sequencing of hot spot exonal areas for c-kit and PDGFRA were done using extracted DNAs of all 90 paraffin block tissues. RESULTS: Among the 90 cases, 83.3% (75/90) were c-kit positive, 16.6% (15/90) were c-kit negative, 93.3% (84/90) were PDGFRA positive, and 6.6% (6/90) cases were PDGFRA negative. Fifteen cases of c-kit negative GIST included 1 case of PDGFRA negative and 5 cases of PDGFRA negative GIST were ckit positive. The one case in which both c-kit and PDGFRA were negative, showed a c-kit mutation in exon 11. CONCLUSIONS: Combined immunohistochemical staining of c-kit, discovered on GIST 1 (DOG1) and PDGFRA is helpful for the diagnosis of GIST. When all staining tests are negative for immunoreactivity, c-kit mutation analysis for exon 11, 9 should be done. Genotyping of kit and PDGFRA do not need to be examined initially, if it is only for the diagnosis of GIST.
