Roles of chloride channels in apoptosis induced by adriamycin in nasopharyngeal carcinoma cells
10.3969/j.issn.1001-1978.2015.09.014
- VernacularTitle:氯通道在阿霉素诱导鼻咽癌细胞凋亡中的作用
- Author:
Mei LIU
;
Hai LUO
;
Jiawei LIN
;
Yan WEI
;
Yuan LI
;
Shanwen LIU
;
Long MENG
;
Lili ZOU
;
Linyan ZHU
;
Liwei WANG
;
Lixin CHEN
- Publication Type:Journal Article
- Keywords:
adriamycin;
nasopharyngeal neoplasms;
chloride channels;
patch clamp technique;
apoptosis;
apoptotic volume decrease
- From:
Chinese Pharmacological Bulletin
2015;(9):1249-1253
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the roles of chloride channels in the apoptosis and apoptotic volume de-crease (AVD)induced by adriamycin in nasopharyn-geal carcinoma CNE-2Z cells.Methods Apoptotic rates were detected by flow cytometry,and the volume changes were measured by the time-lapse live cell ima-ging technique.The patch clamp technique was used to record whole-cell chloride currents.Results Adria-mycin induced apoptosis of CNE-2Z cells.An early ap-optotic volume decrease was observed in the cell trea-ted with adriamycin.The cell volume was decreased by about 10% in 2 h.Adriamycin activated a chloride current which showed outward rectification.The chlo-ride channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB ) could inhibit the adriamycin-in-duced chloride currents,apoptosis and prevent cell shrinkage.Conclusions Our findings suggest that ad-riamycin causes cell apoptosis by activation of chloride channels.Chloride channels may be involved in the apoptosis and apoptotic volume decrease induced by adriamycin in CNE-2Z cells.
