Significance study of SHP2 Expression in brain tissue of bacterial meningitis rat
10.3760/cma.j.issn.1008-6706.2015.17.029
- VernacularTitle:细菌性脑膜炎大鼠脑组织中蛋白络氨酸磷酸酶表达及意义
- Author:
Qiang WANG
;
Xijuan GU
- Publication Type:Journal Article
- Keywords:
Bacterial meningitis;
SHP2 expression
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(17):2646-2648
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the significance of protein tyrosine phosphatase (SHP2)expression in bac-terial meningitis.Methods 90 rats were divided into meningitis group (72)and healthy controls (18)two groups based on the random number table,The SHP2 expression in rat brain tissue at different time points of meningitis group and healthy control group were tested by reverse transcription (RT)a PCR,Western blotting,immunohistochemical methods,then the relationship between SHP2 protein expression and tumor necrosis factor-α(TNF-α),white blood cell (WBC)counts were observed and analyzed.Results The cortical SHP2 mRNA expression of meningitis rat in-cluding (0.035 ±0.020),(0.200 ±0.049),(0.129 ±0.032)and (0.057 ±0.039),were significantly higher than those of the healthy control group (0.031 ±0.028)(F=12.74,P<0.05);the third periventricular SHP2 positive cells were significantly more than those of the healthy control group (χ2 =5.02,P<0.05);the correlation coefficient of cortical SHP2 protein expression and concentration of CSF TNF -α,the correlation coefficient of cortical SHP2 protein expression and WBC counts were respectively 0.08(P>0.05)and 0.77 (t=4.303,P<0.05).Conclusion SHP2 participates in pathophysiology of bacterial meningitis,the main role may be in suppressing inflammation and repairing inflammatory response,it can be used as a reference indicator of condition changes.
