Homing ability of the SCA-1+/CD45+/CD31+ subgroup of bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2015.41.003
- VernacularTitle:骨髓间充质干细胞SCA-1+/CD45+/CD31+亚群的归巢能力
- Author:
Jigang HE
;
Hongrong LI
;
Longsheng GUI
;
Yongwu LI
;
Dan YAN
;
Ping WANG
- Publication Type:Journal Article
- Keywords:
Myocardial Infarction;
Bone Marrow;
Mesenchymal Stem Cels;
Cel Movement;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(41):6572-6578
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Since the FDA was the first to approve autologous bone marrow stem cel transplantation for treatment of myocardial infarction in 2003, there has a large number of clinical and basic research reports. However, their conclusions are different and stem cel homing is a key point. OBJECTIVE:To explore the homing abilities of different subgroups of mouse bone marrow mesenchymal stem cels in myocardial regeneration. METHODS:After mouse bone marrow mesenchymal stem cels were detected using a mouse cardiac stem cel surface differentiation antigen, four cel subgroups were separated on the basis of CD45 and CD31. The homing abilities of the four subgroups were assayed in a Transwel chamberin vitro. The different cel subgroups were injected into the model mice suffering from myocardial infarction for 48 hours. The mice were sacrificed at 48 hours, 96 hours, and 7 days after injection; the hearts were taken and analyzed through whole-body imaging and fluorescence intensity detection. RESULTS AND CONCLUSION:The SCA-1+/CD45+/CD31+ subgroup exhibited the strongest homing ability. The whole-body imaging indicated that the fluorescence intensity of SCA-1+/CD45+/CD31+ subgroup was higher than that of the other subgroups at 48 hours, 96 hours and 7 days after stem cel injection. The migration rate of SCA-1+/CD45+/CD31+ subgroup was also the highest. These findings indicate that the homing ability of the SCA-1+/CD45+/CD31+ subgroup of mouse bone marrow mesenchymal stem cels exhibit a homing trend to the damaged myocardial tissue.
