PTEN silencing effects on biological properties of bone marrow mesenchymal stem cells

VernacularTitle:PTEN基因沉默对骨髓间充质干细胞生物学特性的影响
Author: Yi CHEN ; Xiaodong LU ; Danping LIU ; Guanjun TU
Publication Type:Journal Article
Keywords: Bone Marrow; Mesenchymal Stem Cels; PTEN Phosphohydrolase; RNA Interference; Tissue Engineering
From: Chinese Journal of Tissue Engineering Research 2015;(41):6590-6594
CountryChina
Language:Chinese
Abstract: BACKGROUND:The self-renew and regeneration capacity of the injured spinal cord is thought to be limited. Accordingly, cel transplantation is one potential strategy for promoting functional recovery after spinal cord injury. OBJECTIVE:To explore the effects ofPTEN silencing on the biological properties of bone marrow mesenchymal stem cels, hoping to offer better seed cels for tissue engineering. METHODS:Bone marrow mesenchymal stem cels were transfected with specific siRNA-silencedPTEN gene using the liposome method, and then RT-PCR was used to detect the mRNA expression ofPTEN. Variation of biological properties ofPTEN-transfected cels were detected by the way of MTT assay, cel cycle analysis, and Transwel assay. RESULTS AND CONCLUSION:PTEN is expressed highly in bone marrow mesenchymal stem cels, which is successfuly interfered by siRNA.PTEN-silenced cels have stronger survival, proliferation and migration abilities, which become a kind of better seed cels for tissue engineering.