CD133+ ovarian cancer stem-like cells differentiate into vascular endothelial cells
10.3969/j.issn.2095-4344.2015.41.012
- VernacularTitle:CD133+卵巢癌干细胞样细胞在血管内皮细胞中的分化
- Author:
Liyan JIANG
;
Xiangying LOU
;
Zineng WANG
;
Yanyan LU
;
Ruiping GAO
- Publication Type:Journal Article
- Keywords:
Stem Cels;
Ovarian Neoplasms;
Endothelial Cels;
Cel Differentiation;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(41):6623-6627
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Numerous studies have confirmed that neovascularization plays an important role in the growth, invasion and metastasis of tumors. OBJECTIVE:To investigate the features of CD133+ ovarian cancer stem-like cels differentiating into vascular endothelial cels. METHODS:CD133+ ovarian cancer stem-like cels were successfuly harvested from A2780 ovarian cancer cel lines using serum-free culture method, and incubatedin vitro onto 96-wel plates with or without Matrigel. Then, we observed the capacity of CD133+ ovarian cancer stem-like cels and human umbilical vein endothelial cels to form tube-like structures at different time points. Through xenograft experiments, the role of CD133+ ovarian cancer stem-like cels in the angiogenesis of ovarian cancer was observed using immunofluorescence staining. RESULTS AND CONCLUSION:CD133+ovarian cancer stem-like cels and human umbilical vein endothelial cels cultured with no Matrigel had no corresponding lumen formation, and could not express CD31. But those cultured with Matrigel had lumen formation and expressed CD31 significantly. After tumor formation, human-derived CD31 expression was observed in the tumors. These findings indicate that CD133+ ovarian cancer stem-like cels can differentiate into vascular endothelial cels, and be involved in tumor revascularization.
