miR-486 is a tumor suppressor in glioma stem cells
10.3969/j.issn.2095-4344.2015.41.014
- VernacularTitle:miR-486对胶质瘤干细胞的抑制作用
- Author:
Jing ZHOU
;
Baochao ZHANG
- Publication Type:Journal Article
- Keywords:
Brain Neoplasms;
Glioma;
MicroRNAs;
Cel Cycle;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(41):6633-6637
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous studies have found that the expression level of miR-486 in glioma stem cels (CD133+) is significantly down-regulated compared with that in glioma non-stem cels (CD133-), but the effect of down-regulation of miR-486 on CD133+ cels remains unclear . OBJECTIVE: To explore the effect of miR-486 on CD133+ cels. METHODS:CD133+ glioma stem cels and CD133- glioma cels were separated from U87 cels by flow cytometer. miR-486 overexpression glioma stem cels were constructed by lipofection transfection. RESULTS AND CONCLUSION:After sorting and purification, the content of the CD133+ fraction was enriched up to 83.5%. The expression level of miR-468 in CD133+ glioma stem cels was obviously down-regulated compared with that in CD133- glioma cels. CD133+ glioma stem cels overexpressing miR-486 were fabricated successfuly. Results from in vitro experiments showed that miR-486 overexpression could dramaticaly decrease the proliferation of glioma stem cels, induce a cel cycle arrest in G1/S phase for CD133+ glioma stem cels and promote cel apoptosis. These findings suggest that miR-486 can be a suppressor of glioma stem cels, which offers a novel potential therapeutic target for glioma stem cels and human glioma.
