Non-alcohol fatty liver disease reduces the intestinal barrier function of rats

VernacularTitle:非酒精性脂肪肝大鼠肠道黏膜屏障功能的实验研究
Author: Tao LIU ; Qiuling XU ; Yan ZHAO
Publication Type:Journal Article
Keywords: Non alchohol fatty liver disease; Intestinal barrier function; Zonula occludens-1; Occluding
From: Journal of Medical Postgraduates 2015;(11):1124-1127
CountryChina
Language:Chinese
Abstract: Objective Disorder of intestinal barrier function is associated with the non-alcohol fatty liver disease ( NAFLD) . The present paper aimed to explore the intestinal barrier function in the rat model of NAFLD . Methods Sixteen SD rats were ran-domly divided into an NAFLD and a normal control group of equal number .The NAFLD models were constructed by high-fat feeding . HE staining was used for pathologic examination of the liver , the levels of TNF-α, IL-1, and endotoxin (ET ) were measured by ELISA and the limulus reagent method , and the expressions of intestinal ZO-1 and Occluding were determined by real time PCR . Resu lts Compared with the normal controls , the NAFLD rats showed typical hepatic lipid deposition , with significantly increased levels of serum TNF-αand IL-1 and plasma ET and decreased expressions of intestinal ZO-1 and Occluding (P<0.05). Conclusion Intestinal barrier function is decreased in NAFLD rats .