Comparative analysis of G test on diagnosis of deep fungal infection by colormetric and turbidimetric meas-urements
10.16571/j.cnki.1008-8199.2015.11.014
- VernacularTitle:G试验显色法与浊度法对深部真菌感染诊断的比较分析
- Author:
Shuang LIU
;
Longqin CHEN
;
Lin CONG
;
Qiong MIAO
;
Lin LIU
;
Yang SONG
- Publication Type:Journal Article
- Keywords:
G test;
Coloration method;
Turbidimetric measurement;
Deep fungal infection
- From:
Journal of Medical Postgraduates
2015;(11):1175-1178
- CountryChina
- Language:Chinese
-
Abstract:
Objective Not much information is available on the comparative analysis of G test on diagnosis of deep fungal in -fection by colormetric and turbidimetric measurements .The purpose of this paper was to explore the clinical value of fungal (1-3)-β-D-glucan detection kit ( colormetric measurement ) . Methods 89 clinical samples collected from Hainan Branch of PLA General Hos-pital were detected by fungal (1-3)-β-D-glucan detection kit (turbidimetric measurement) and fungal (1-3)-β-D-glucan detection kit ( colormetric measurement ) respectively , among which 32 cases were from disease group ( deep fungal infection in patients ) and 57 ca-ses were from control group ( healthy person ) .The comparison was made on the sensitivity , specificity and accuracy of these two meth-ods. Results The sensitivity, specificity and accuracy of colormetric measurement kit on the diagnosis of deep fungal infection were obviously higher than those of turbidimetry method kit (81.2%vs 53.1%, 91.2%vs 75.4%, 87.6%vs 67.4%, P<0.05), which was of significant difference .Detection result of colormetric method had a positive coincidence rate with other systems '( except respira-tory system) deep fungal infection, which was obviously higher than turbidimetry method (92.8%vs 57.1%, P<0.05).Positive co-incidence rate and total coincidence rate between colormetric method kit and clinical diagnosis result on differentiate samples were obviously higher than those of turbidimetric method kit (P=0.01). Conclusion Colormetric measurement kit has higher accuracy and higher coincidence rate with clinical diagnosis than turbidimetry meas-urement kit , which is better for clinical service .
