The expression of hsa-miR-28-3p and ADAM12 in cord blood mononuclear cells of preterm birth in-fants
10.3760/cma.j.issn.1673-4912.2015.10.006
- VernacularTitle:早产儿脐带血单核细胞中 hsa-miR-28-3p和 ADAM12的表达
- Author:
Yunlin SHEN
;
Xiaohui GONG
;
Hong LI
;
Jingjing SUN
;
Gang QIU
;
Nan ZHONG
- Publication Type:Journal Article
- Keywords:
Preterm birth;
MicroRNA;
hsa-miR-28-3p;
ADAM12
- From:
Chinese Pediatric Emergency Medicine
2015;22(10):689-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective Preterm birth is a live birth delivered before 37 weeks of gestation.Preterm birth rates have risen in recent years,and preterm birth is the leading cause of perinatal morbidity and mortali-ty worldwide.Both environmental and genetic factors likely play important roles in the development of pre-term birth.MicroRNAs(miRNAs)are 18 to 25 nucleotides,single stranded non-coding RNAs that regulate a wide range of biological processes in development and human disease.In this study,we have investigated the differential expression of hsa-miR-28-3p and ADAM12 in cord blood mononuclear cells of preterm birth and term birth.Methods The cord bloods were collected from the patients of Putuo District Institute of Materni-ty and Child Health.The preterm group(30 patients)was the patients with spontaneous preterm delivery,and the control group(30 patients)was the patients delivered normal infants at term.The expression levels of hsa-miR-28-3p and ADAM12 mRNA were directly generated by real-time PCR.Results The gestational age and birth weight of preterm group was(30.92 ±1.73)weeks and(1 646 ±357)g,and the control group was (39.73 ±0.58)weeks and(3 301 ±394)g.The hsa-miR-28-3p expression of preterm group(1.03 ±0.23) was significantly lower than that of control group(2.32 ±0.52)in the peripheral blood mononuclear cells (P <0.01 );the ADAM12 mRNA expression of preterm group(0.037 8 ±0.005 6)was significantly higher than that of control group(0.027 6 ±0.003 9)(P <0.05);the hsa-miR-28-3p expression was significantly correlated with the ADAM12 mRNA expression(r =-0.634 1 ,P <0.01 ).Conclusion The lower hsa-miR-28-3p expression of cord blood mononuclear cells may up-regulate ADAM12 mRNA expression,and promotes the occurrence of spontaneous preterm birth.
